human ace2 pe conjugated antibody (Bio-Techne corporation)
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Human Ace2 Pe Conjugated Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human ace2 pe conjugated antibody/product/Bio-Techne corporation
Average 94 stars, based on 2 article reviews
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1) Product Images from "Preventive treatment of coronavirus disease-2019 virus using coronavirus disease-2019-receptor-binding domain 1C aptamer by suppress the expression of angiotensin-converting enzyme 2 receptor"
Article Title: Preventive treatment of coronavirus disease-2019 virus using coronavirus disease-2019-receptor-binding domain 1C aptamer by suppress the expression of angiotensin-converting enzyme 2 receptor
Journal: Journal of Advanced Pharmaceutical Technology & Research
doi: 10.4103/JAPTR.JAPTR_117_23
Figure Legend Snippet: Spike pseudotyped lentiviral particles infect A549-hACE2 cells. Spike D614 (a) and delta B-1.6172 (b) pseudotyped GFP lentiviral particles infect 2 × 104 A549-hACE2 cells. GFP: Green fluorecent protien
Techniques Used:
Figure Legend Snippet: Dose–response curves of the CoV-2-RBD-1C aptamer’s IC 50 . 0.05, 0.15, 0.32, 0.75, 1.56, 3.12, 6.25, 12.5, 25, 50, and 100 μM dose concentrations of CoV-2-RBD-1C aptamer were applied to A549-hACE2 cells for 72 h. Through the use of nonlinear regression analysis, IC 50 values were calculated (Prism Pad 8.1). Results are based on the triplicate of the data, IC 50 : Half-maximum inhibitory concentration, CoV-2-RBD-1C: Coronavirus-2-receptor-binding domain 1C, CoV-2-RBD-1C: Coronavirus-2-receptor-binding domain 1C
Techniques Used: Concentration Assay, Binding Assay
Figure Legend Snippet: CoV-2-RBD-1C aptamer effect son SARS-CoV-2-Spike-ACE2 inhibition. The ELISA method was used to measure the binding inhibition. Bars refer to mean ± SD. Distilled water is the solvent control; * P < 0.05. One-way ANOVA was used to determine the difference’s significance with the solvent control. SARS-CoV-2: Severe acute respiratory syndrome coronavirus-2, ACE2: Angiotensin-converting enzyme 2, SD: Standard deviation, CoV-2-RBD-1C: Coronavirus-2-receptor-binding domain 1C
Techniques Used: Inhibition, Enzyme-linked Immunosorbent Assay, Binding Assay, Solvent, Standard Deviation
Figure Legend Snippet: Preincubation with the aptamer CoV-2-RBD-1C prevents the spike D614 binding and its variants N501Y and D614G to human A549-hACE2 cells. One-way ANOVA was employed to evaluate the significant variations in contrast to the related solvent control. * P < 0.05; bars represent mean ± SD. SD: Standard deviation, CoV-2-RBD-1C: Coronavirus-2-receptor-binding domain 1C
Techniques Used: Binding Assay, Solvent, Standard Deviation
Figure Legend Snippet: The ability of viruses to transduce A549-hACE2 cells was inhibited by the CoV-2-RBD-1C aptamer. * P < 0.05; n more than or equal to 3 independent tests. One-way ANOVA was employed to evaluate the significance of the difference with the solvent control. CoV-2-RBD-1C: Coronavirus-2-receptor-binding domain 1C
Techniques Used: Transduction, Solvent, Binding Assay
Figure Legend Snippet: Ability of viruses to transduce A549-hACE2 cells was inhibited by the CoV-2-RBD-1C aptamer. (a) Before being infected with the SARS-CoV-2 spike Delta B-1-617-2 variants 7500 TU/mL for 24 h. * P < 0.05; n more than or equal to 3 independent tests. one-way ANOVA was used to evaluate the significance of the difference in relation to the solvent control. SARS-CoV-2: Severe acute respiratory syndrome coronavirus-2, CoV-2-RBD-1C: Coronavirus-2-receptor-binding domain 1C
Techniques Used: Transduction, Infection, Solvent, Binding Assay